Technical Assistant*in (d/f/m) Translational Immunology

Technical Assistant (d/f/m) Translational Immunology

Das Wichtigste in Kürze

• Arbeitszeiten

  Vollzeit

• Eintrittsdatum

• Dauer der Anstellung

  Befristet

• Bewerbungsfrist

• Kennziffer

  4112

• Entgeltgruppe

Company describtion

The mission of the Berlin Institute of Health at Charité (BIH) is medical translation: transferring biomedical research findings into novel approaches to personalized prediction, prevention, diagnostics and therapies and, conversely, using clinical observations to develop new research ideas. The aim is to deliver relevant medical benefits to patients and the population at large. Since 2021 the BIH has been integrated into Charité as its so-called third pillar.

The Sawitzki lab at the Berlin Institute of Health at Charité – Universitätsmedizin Berlin investigates the molecular mechanisms of immune cell activation and regulation to reveal how components of the immune response themselves become a trigger of destructive inflammatory reactions, like in the context of autoimmune disease or post-acute infections (post-COVID19). We are using state-of-the-art multi-omics technologies (cytometry, imaging, scRNAseq) as well as in vitro perturbation cultures to dissect causalities behind compositional changes of immune cells. We are an international research group and closely involve our technical assistants in our research projects. We are looking for a highly research-interested, motivated and flexible technical assistant for our new BMBF-funded research project “CURE-ME” to support the analysis of the immune cells in Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS).

What you can expect

The candidate will support the scientific staff in a project that aims to understand the link between EBV, EBV-related and other autoantigens as drivers of aberrant immune cell responses and autoimmunity in ME/CFS.

This includes the following tasks:

• Preparation of patient samples from human blood (e.g. PBMCs, serum, whole blood)
• Preparation of patient immune cell samples for cytometric analysis including antibody preparation and coupling, staining of patient samples following standard protocols and measurements using flow or spectral cytometric analysis (or coordination with core facility for mass cytometry)
• Preparation of patient samples and measurements using ELISA or Milliplex assay
• Performance of in vitro assays following standard protocoll
• You will work closely with international basic and data scientists in the execution of the project. Excellent communication and teamwork skills are key to rapid progress and success.

What you bring along

• Education/training as lab technician or technical assistant (BTA, MTA, biology laboratory technician or comparable) or a Bachelor (B.Sc.)/Master (M.Sc.) degree in natural sciences, both with long-time practical experience in an immunological laboratory
• Experience in preparing patient samples from blood and tissue and in cell culture of primary cells
• Profund experience in performing  flow cytometric and  mass cytometric measurements (more than 40 markers) in coordination with scientific staff
• Profund experience in performing ELISA and Milliplex measurements
• Experience in bioinformatic analysis on the basis of established protocols /pipelines in R in coordination with scientific staff
• Structured, independent, and careful way of working, e.g. ability to implement a variety of laboratory techniques using standard protocols after a thorough training by our scientific staff members
• Excellent spoken and written German and English

What we offer you

• A varied job in a forward-looking research institute
• Pay group E9b TVöD VKA-K. The classification is based on qualifications, the respective experience level is calculated on the basis of professional experience. The annual salary (gross) is stated for a full-time position without special or additional payments. The collective agreement can be found here.
• Additional benefits customary in the public sector (including annual special payment, company pension scheme (VBL), capital-forming benefits)
• Flexible working hours and the option of working remote
• 30 vacation days per year (with a five-day week)
• Various support offers to balance work and family life (childcare, cooperation with voiio)
• Very good training and further education opportunities
• Mobile citizens' office on site
• Corporate benefits (travel, leisure, shopping, etc.), Charité Gympass, bicycle leasing or abonnement (JobRad, mylo)
• Very easily accessible and attractive workplace at the Rahel Hirsch Center for Translational Medicine, Luisenstr. 65, 10117 Berlin

We live diversity!

Note: If you have a foreign degree, please submit proof of recognition of your degree in Germany with your application. The proof can be obtained via the anabin database. Please note that it may be necessary to obtain a certificate assessment from the ZAB. You can find more information here.

Proof of measles immunity / measles vaccination is a prerequisite for employment for those born after 1970.

You can find more information about BIH here.

Contact person

If you have any technical questions about the job posting, please contact Dr. Manuela Fiedler (ma*****.*******er@bi*-*******te.de).